茶刺蛾核型多角体病毒与其它杆状病毒关系的基因分析

Analysis of AcORF47 Homologue and VP80 Gene from a Newly Isolated Nucleopolyhedrovirus from the Tea Slug Moth, Iragoides fasciata

茶刺蛾核型多角体病毒Iragoides fasciata Nucleopolyhedrovirus(IrfaNPV)是新分离的一种杆状病毒.本研究克隆和分析了IrfaNPV的AcORF47 同源基因和VP80基因.AcORF47同源基因与苜蓿丫纹夜蛾多粒包埋型核型多角体病毒(Autographa californica multiple nucleocapsid nucleopolyhedrovirus,MNPV,AcMNPV)的ORF47和家蚕核型多角体病毒(Bombyx mori nucleopolyhedrovirus,BmNPV)的ORF38有42%的同源性,与薄荷灰夜蛾多粒包埋型核型多角体病毒(Rachiplusia ou multiple nucleocapsid nucleopolyhedrovirus,RaouMNPV)的ORF44有40%的同源性.IrfaNPV的VP80 C端222氨基酸与17种NPV特有结构蛋白VP80/P87有25%~58%同源性.在18种VP80/P87分子进化树上,IrfaNPV与AcMNPV、BmNPV和RaouMNPV的亲源关系最为接近.

A baculovirus, Iragoides fasciata Nucleopolyhedrovirus(IrfaNPV)was newly isolated from the tea slug moth, Iragoides fasciata. The IrfaNPV AcORF47 homologue and VP80 gene were cloned. Sequence analysis showed IrfaNPV AcORF47 homologue shared 42%, 42% and 40% identities with AcMNPV ORF47, BmNPV ORF38 and RaouMNPV ORF44 in amino acid level, respectively. The 222 amino acid residues in the C-terminal of IrfaNPV VP80 had 25%~58% identities with homologous genes from other 17 NPVs. A phylogenetic tree was constructed based on the VP80/P87 amino acid sequences and the result showed that IrfaNPV was most closely related to AcMNPV, BmNPV and RaouMNPV.