人脐静脉内皮细胞体外培养与鉴定新方法探索

Study on Culture and Identification of Human Umbilical Vein Endo thelial Cells by CD105 and CD31

目的 :探讨人脐静脉内皮细胞 (HUVEC)培养与鉴定新方法。方法 :Ⅰ型胶原酶消化、分离HUVEC ,含内皮细胞生长添加物 (12. 5mg/L)、肝素 (10 0mg/L)的体积分数 2 0 %胎牛血清_M199培养系统培养HUVEC。流式细胞术和免疫荧光法检测其endoglin(CD10 5 )、血小板源内皮细胞黏附分子_1(CD31)表达。结果 :HUVEC均表达CD10 5和CD31;HUVECCD10 5 + 百分率为 96 . 5 6 % ,CD31+ 90 . 4 2 %。结论 :该培养系统简便、可行 ,CD10 5和CD31有助于鉴定HUVEC的分离纯度。

Objective:To study on culture and identification of human umbilical vein endothelial cells(HUVEC).Methods:Human endothelia l cells were isolated from umbilical veins,cultured in 20% fetal bovine serum (FBS)_M199 with endothelial cell growth supplement(12.5?mg/L) and heparin(100?mg/L)and identified using endoglin(CD105)、platel et_endothelial cell adhesion molecule_1(CD31)by flow cytometry and immun ofluorescent assay.Results:CD105 and CD31 in HUVEC were both positive expressio n.The percentage of HUVEC by flow cytometry was 96.56% in CD105+ and 90.42% in CD31+.Conclusion:The culture system for HUVEC is practical.CD105 and CD31 are helpful for identification of HUVEC.