原因不明习惯性流产患者主动免疫前后细胞免疫功能的变化

Changes in T-lymphocyte and Natural Killer Cell Subsets before and after Active Immunotherapy for Patients with Unexplained Habitu al Abortion

目的 :探讨细胞免疫功能的变化与原因不明习惯性流产 (UHA)的发生及主动免疫治疗机制的关系。方法 :用流式细胞仪测定并比较 6 5例UHA患者和 30例正常已生育妇女 (NF组 )及 30例经主动免疫治疗后的UHA患者外周血CD3+、CD4 +、CD8+、CD16 +CD5 6 +细胞亚群比例及CD4 +/CD8+的比值 ;同时比较采用主动免疫治疗 30例和未采用主动免疫治疗的 35例UHA患者再次妊娠成功率。结果 :UHA患者与NF组比 ,血中CD3+、CD16 +CD5 6 +细胞的百分率及CD4 +/CD8+比值增高 ,差异有统计学意义 (P <0 . 0 5 )。主动免疫后CD3+、CD16 +CD5 6 +细胞百分率及CD4 +/CD8+比值下降 (P<0 . 0 5 )。主动免疫治疗组的再次妊娠成功率为 92 . 86 % ,未主动免疫治疗组 2 9 .0 3% ,两组比较P <0 . 0 1。结论 :淋巴细胞亚群比例的改变与UHA的发生有关 ,主动免疫治疗可调节异常的细胞免疫功能 ,有利于再次妊娠成功率的提高。

Objective:To explore the relation between the changes in cellular immunity and t he causes of unexplained habitual abortion(UHA)and the mechanism of acti ve immunotherapy on UHA patients.Methods:T-lymphocyte and natural killer ( NK)cell subsets of peripheral blood were detected by flow cytom etry and compared in 65 cases of UHA and 30 cases of normal fertile d(NF)women as well as 30 cases of UHA after active immunotherapy.The ne xt successful pregnancy rates of UHA patients with and without active immunother apy were compared.Results:There were no differences in the percentages of CD4+ a nd CD8+ cells between UHA cases and NF cases,however the percentages of CD 3+ and CD16+CD56+ cells as well as the ratio of CD4+/CD8+ of UHA cases were significan tly higher than those of NF cases (P<0.05).After active immunoth erapy,the percentages of CD3+ and CD16+CD56+ cells as well as the ratio of CD4+/ CD8+ of UHA cases were lower significantly(P<0.05).However there w ere no differences in the percentages of other T-lymphocyte subsets.The success ful pregnancy rates of UHA cases with and without active immunotherapy were 92. 86% and 29.03% respectively.The difference was significant(P<0.05) .Conclusion:The changes in T-lympyocyte and NK cell subsets have something to d o with UHA.Active immunotherapy can regulate the cell immunization function and increase the next successful pregnancy rate effectively.