牙龈卟啉菌特异基因片段的体外扩增和鉴定

Amplification and identification of the specific gene fragment of porphyromonas gingivalis in vitro

Ｐｇ与口腔感染性疾病关系密切．现根据Ｐｇ特异的菌毛亚单位蛋白结构基因设计寡聚核苷酸引物，采用ＰＣＲ技术扩增了其中长５４２ｂｐ的片段并对其进行了特异性鉴定。结果表明：所设计的引物能从５株Ｐｇ菌中扩增出大小一致的特异ＤＮＡ片段，但不能从其它１８株异种菌中扩增出产物，故具有高度的种特异性．其检测的敏感性为０．ｌｎｇ，为ＰＣＲ应用于口腔致病菌的检测奠定了基础。

Porphyromonas gingivalis(Pg) has been associ.ated with some oral infectious diseases. In the present study, the oligodeoxynucleotide primers were designed to amplify specifically a segment of the subunit protein fimbrial gene of Pg. The specific product of 542-bp was amplified by polymerase chain reaction(PCR). The results showed that 5 strains of Pg tested yielded specific DNA fragments of identical size, while 18 strains of heterologous species failed. to amplify specific products, and demonstrated high species-specificity of the primer.The sensitivity of the primers reached 0. lng. Tlie results are of practical value for the detection of oral pathogenes by PCR.