冻融前后人牙髓等成纤维细胞生物学特征的比较

The effects of cryopreservation on cultured fibroblast cells from human pulp, periodontal ligament and gingiva in the aspect of biology

本研究对体外培养的第5代人牙髓牙周牙龈成纤维细胞进行了冷冻和复苏,三种细胞复苏的存活率分别为14%、19%和24%,复苏后细胞的生长曲线和倍增时间等生物学特征与冻存前基本一致,提示冻融后的人牙髓牙周牙龈成纤维细胞基本保持冻融前的生物学特征.

In this study, we thawed the fifth passage cells preserved in liquid nitrogen.The results of the cryoprescrved flbroblast cells illustrated: the success rates of thawed pulpal. periodontal and gingival cells were 14%,19% and 24% respectively, there was no significant difference between frozen cells and non梖rozen cells on growth and proliferation.