牙周炎患者龈沟液和龈组织中IL—8的测定

Measurement of interleukin-8 in gingival crevicular fluid and gingival tissue of periodontal disease.

采用ELISA间接夹心法测定了23名成人牙周炎患者34个患牙龈沟液中的IL-8.并用免疫组织化学法对相应的炎性龈组织进行检测.结果表明IL-8与牙周炎症密切相关,牙周炎龈沟液(GCF)中IL-8检出率为88.24%.IL-8含量范围为0.006～2.772ng/μl.其含量与牙龈指数相关非常显著,与牙周袋深度相关显著,与附着丧失、菌班指数无明显相关.炎性龈组织切片染色表明龈上皮组织和胶原组织中含有大量的阳性染色,从组织学上证明了GCF中IL-8的来源.提示IL-8可能在牙周炎发病机制中起着重要作用.

Gingival crcvicular fluid(GCF) from pcriodontal disease were found to contain from 0.006ng/μl to 2.772 ng/μ1 intcrlcukin-8(IL-8) as determined by a sensitive enzyme linked immunoassay.No IL-8 was detected in saliva and normal GCF, IL-8 in GCF of pcriodontal disease was related to the number of probing depth and bleeding upon probing but was unrelated to the number of clinical attachment level and plaque index. Inflamed gingival tissues were examined b combing immunohistochcmistry, IL-8 was observed both in fibrillate tissue and epithelium.These results demonstrate that TL-8 may play an important role in the periodontal disease.