托吡酯对沙土鼠脑缺血再灌注损伤保护作用的研究

Protective effects of topiramate on cerebral ischemia-reperfusion damage of pallasiomies

目的 探讨托吡酯对脑缺血再灌注所致脑损伤的保护作用及机制。方法 取沙土鼠 4 0只 ,随机分为 :⑴假手术组 ;⑵脑缺血再灌注组 ;⑶缺血再灌注 +托吡酯 5 0mg·kg-1组 ;⑷缺血再灌注 +托吡酯 10 0mg·kg-1组。采用短暂夹闭双侧颈总动脉法复制沙土鼠脑缺血再灌注模型 ,术后 2 4h取脑组织测定SOD、MDA、NO的改变 ,以及含水量的变化。结果 托吡酯 5 0mg·kg-1、托吡酯 10 0mg·kg-1脑组织中SOD活力明显增高、MDA、NO含量明显减少、脑组织含水量明显降低 ,与缺血再灌注组比较均有显著性差异 (P <0 0 1)。结论 托吡酯能使脑缺血再灌注损伤后脑组织中自由基的含量明显减少 ,能明显减轻脑组织水肿 ,对脑缺血再灌注损伤有保护作用。

Objective: To investigate the protective effect and mechanism of topiramte against the cerebral damage caused by ischemia-reperfusion. Methods: Forty pallasiomies were randomly divided into four groups: ⑴sham operation; ⑵ischemia-reperfusion; ⑶ischemia-reperfuison and 50 mg·kg -1 topiramte and; ⑷ischemia-reperfuison and 100 mg·kg -1 topiramte. Cerebral ischemia-reperfusion replicas were made by clipping bilaterial common carotidarteries after seven minutes in the second, the third and the fourth groups, but not in the sham operation group. Twenty four hours after the operation, the pallasiomies were killed. The contents of SOD,MDA,NO and water in cerebral tissue were measured. Results: In the groups of topiramte prevention, the activity of SOD was higher, the contents of MDA, NO and water were lower, and there is significant difference compared to that of ischemia-reperfusion group (P<0.01). Conclusion: Topiramte can obviously reduce contents of free radicals and cerebral edema caused by ischemia-reperfusion and has protective effects against ischemia-reperfusion.