大鼠海马CA1区锥体神经元树突分支的生后发育

POSTNATAL DEVELOPMENT OF DENDRITIC ARBORIZATION IN RAT HIPPOCAMPAL CA1 PYRAMIDAL NEURONS

目的 研究大鼠海马CA1区锥体神经元树突分支的生后发育。 方法 应用biocytin细胞内染色方法。 结果 CA1锥体神经元的树突在生后第 2～ 3周发育最快。顶树突的分支和长度在生后 2 1d发育成熟 ;而基树突要到生后 5 6～ 70d才发育成熟。基树突的发育较顶树突慢。 结论 细胞内染色技术可更完整地显示神经元的形态。海马CA1区锥体神经元在出生后继续发育 ,且基树突的发育较顶树突慢。

Objective To investigate the postnatal development of dendritic arborization in rat hippocampal CA1 pyramidal neurons. Methods Biocytin intracellular staining method was used in present study. Results The dendritic arborization of CA1 pyramidal neurons developed rapidly during the postnatal 2-3 weeks.The apical dendritic arborization reached the adult state at P21,whereas the basal dendritic arborization did not mature until P56-70.Furthermore,the basal dendrite developed significantly more slowly than the apical dendrite.Conclusion The neuronal morphology can be visualized much completely by intracellular staining method.The hippocampal CA1 pyramidal neurons continue to grow after birth,and the basal dendrite matures more slowly than the apical dendrite.