发育期小鼠肾小体细胞凋亡的研究

STUDY ON APOPTOSIS IN DEVELOPING RENAL CORPUSCLES OF MOUSE

目的 研究小鼠肾小体发育过程中的细胞凋亡规律及形态学特点。 方法 应用光镜、电镜技术和脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记法 (TUNEL法 )分别对不同胚龄和生后日龄小鼠肾小体内细胞凋亡进行观察。 结果 胚龄 14d肾小体发生时就出现细胞凋亡 ,胚龄 18d左右达到高峰 ,随后缓慢下降。肾小体内凋亡细胞以内皮细胞和足细胞多见。凋亡细胞的两种结局 :1 被邻近的细胞所吞噬 ;2 落入肾小体的毛细血管腔或肾小囊中。 结论 小鼠肾小体的整个发育过程均存在细胞凋亡现象 ,肾小体内细胞凋亡与肾小体发育的完善有关。

Objective To study apoptosis and morphological characteristics in the developing renal corpuscles of mouse kidney. Methods Light,transmission electron microscope and TUNEL technique were used to observe apoptosis in the developing renal corpuscles of different embryonic and postnatal mice. Results Apoptotic cells could be found when renal corpuscles occurred at embryonic day 14(E14).It peaked around E18 and decreased thereafter.Electron microscope revealed that apoptotic cells had morphological characteristics such as margination of condensed nuclear chromatin,shrinking cytoplasm.Apoptosis of endothelial cells and podocytes were more frequent.Two results of apoptotic cells were observed,1. being ingested by neighboring cells; 2. apoptotic cells were dropping into glomerular capillary lumens or Bowman capsules.Conclusion Apoptotic cells were found in all the time during the development of mouse renal corpuscles.It might play an important role in the development of renal corpuscles.