APA微胶囊免疫隔离生物膜制备中的质量控制

Quality Control in Preparation of APA Microcapsular Immune Isolation Biomembrane

为了优化制备条件, 简化工艺, 控制质量, 本文观测了APA(海藻酸盐 聚赖氨酸 海藻酸盐)微胶囊免疫隔离生物膜制备过程中部分物理因素和化学因素与质量的关系。结果表明: 微囊的粒径与静电微囊发生仪脉冲频率呈负相关; 与推进泵速度呈正相关; 与针头内径呈正相关。随着聚赖氨酸浓度的增加, 膜厚度显著增加。微囊的粒径不随聚赖氨酸成膜反应时间延长而改变, 但影响膜的厚度。柠檬酸钠的液化时间对微囊的粒径与厚度均无明显影响。通过优化制备条件, APA微胶囊免疫隔离生物膜将具有更好的通透性、柔韧性、生物相容性和强度。

For optimization of preparation condition, simplification of technique and quality control, we observed partial physical and chemical factors in process of preparation of alginate-polylysine-alginate(APA) microcapsular immune isolation biomembrane.The result showed that granular diameter of the microcapsules was negatively correlated with pulse frequency of static microcapsule generator and positively correlated with speed of driving pump and bore of pinhead.The membrane thickness was remarkably increased with the increase of polylysine density.Prolonging reaction time of shaping membrane didn′t change granular diameter of the microcapsules, but influenced membrane thickness.Liquefaction time of sodium citrate had no remarkable effect on granular diameter and membrane thickness.APA microcapsular immuno isolation biomembrane would have better permeability, flexibility, biological compatibility and intensity by optimizing preparation conditions.