高效液相色谱法测定双氯灭痛β－环糊精包合物生物利用度

Determination of bioavailability of diclofenac sodium β-cyclodextrin inclusion by high performance liquid chromatography

用高效液相色谱法测定了单纯双氯灭痛、双氯灭痛β－环糊精包合物（共沉淀法、冷冻干燥法）三种样品在家兔体内的血浓度，在ＩＢＭ计算机上求算药动学参数。相对生物利用度分别为８４７．５１８％（共沉淀法），８７８．５２３％（冷冻干燥法）．双氯灭痛在体内的分布呈一室模型。

The plasma concentrations of the intact diclofenac sodium (coprecipitation method),diclofenac sodium β-cyclodextrin inclusion compound(coprecipitation method and freeze-dried method) in rabbits were determined by high performance liquid chromatography, and the pharmacokinetcs parameters were calculated using IBM computer.The relativebioavailabilities were 847. 51 8%(coprecipitation method),878.523%(freeze-dried method),respectively. Distribution of diclofenac sodium in the plasma of rabbit showed open one compartment linear model.