摘要
本文介绍用HPLC法测定人血清中吗咯替酯(Ⅰ)及其代谢产物(Ⅱ)的浓度,以二仲丁基-1,3-二硫杂环戊烯-2-亚丙二酸酯(Ⅲ)为内标,血样用乙酸乙酯萃取Ⅰ和Ⅱ,浓缩进样,使用Waters-μ-BondapakC_(18μ)3.9mm×300mm色谱柱,流动相为甲醇-水-36%醋酸(80:19.2:0.8),检测波长为362nm。Ⅱ、Ⅰ和Ⅲ的峰分离度好,保留时间分别为5.8、8.2、12.1min。测定线性范围Ⅰ为0.01~1.0μg/ml,r=0.9997,最低检测限为0.005μg/ml;Ⅱ为0.1~1.00μg/ml,r=0.9975。本文还介绍了6例健康人口服吗咯替酯后,用本法测定Ⅰ和Ⅱ的血清浓度及药代动力学参数。
A RP-HPLC modified method has been developed for th determination of malotilate and its main metabolite in human serum Waters μ-Bondapark C_(18) Column was used. The mobile phase was methanol-water-36% HAc (80:19. 2 :0.8). The eluent was monitored at 362nm. Serum samples were extracted with ethyl acetate. The extract was evaporated to dryness and dissolved in 95% alcohol. The colibration curve is linear in the range of 10~20ng/ml (r=0. 999) for metabolite. The minimal detectable concentration of 5ng/ml of Malotilate was achieved. The recoveries of Malotilate and its main metabolite were found to be 99. 4% and 98. 4% respectively.The RSD within day and day to day were less than 5%.The results of pharmacokinetic study were reported. This method is sensitive. simple. accurate and rapid.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1994年第5期21-24,共4页
Chinese Journal of Pharmaceutical Analysis