用HPLC测定大鼠体液和组织中的环氧司坦

Determination of Epostane in Rat Body Fluid and Tissues by HPLC

本文建立了高效液相色谱法（ＨＰＬＣ）测定大鼠血浆、胆汁、尿、粪和肝组织中的环氧司坦浓度。采用ＹＷＧ－Ｃ（１８）Ｈ（３７）柱，甲醇－０．５％四甲基乙二胺缓冲溶液（８０：２０，Ｖ／Ｖ）为流动相，内标为甲基睾丸素，ＵＶ检测波长２５４ｎｍ。血浆样品用环己烷－异丙醇（９７：３，Ｖ／Ｖ）提取，色谱峰分离完全，方法线性良好，ｒ＝０．９９９８，提取回收率为７７．０±５．８％，方法回收率８８．０±５．６％，日内ＲＳＤ为０．６５％～０．７８％，日间ＲＳＤ为２．０２％～３．７７％；胆汁、尿、粪、肝组织的ｒ值分别为０．９９９７、０．９９９８、０．９９９６、０．９９９８，其平均回收率为６８．５５±５．７％、８８．１７±４．２７％、９２．１７±１．０９％、８１．５５±１．４６％。用本法测定大鼠灌胃（ｉｇ）环氧司坦５０ｍｇ／ｋｇ后的药代动力学，并报道了有关参数。

A HPLC method was established for determining epostane in rat plasma, tissue. bile. Feces and urine. The column was packed with YWG-C_(18)H_(37), 10μm and a mobile phase composed of methanol-0. 5% tetramethylenediamine buffer (80 : 20, V/V) was used. Internal standard was methyltestosterone. The detector was operated at UV 254nm. Plasma samples were extracted with cyclohexane-isopropyl alcohol (97:3, V/V), the peak separation and the calibration curve were good, r=0. 9998. The absolute and relative recoveries of epostane in plasma were 77.0±5. 8% and 88.0±5. 6% respectively. The RSD of within-day and day to day were 0. 65%～0. 78% and 2. 02～3. 77% respectively. The correlation coefficients for bile. urine.feces and liver were 0. 9997, 0. 9998. 0. 9996 and 0. 9998, respectively. The average recoveries were 68. 55±5. 7%, 88. 17±4. 27%, 92.17±1. 09% and 81. 55±1. 46% respectively.The method was used in pharmacokinetic studies of epostane in rat after ig 50 mg/kg and its pharmacokinetic parameters were reported.