过量酒精摄入对大鼠细胞因子及胰岛的影响

IL-1β、TNF-α and IL-6 caused by chronic alcohol administration on pancreaticislets of rats

目的 研究长期酒精摄入对大鼠胰岛功能的影响 ,以及白细胞介素Iβ(IL - 1β)、肿瘤坏死因子α(TNF -α)、白细胞介素 6 (IL - 6 )在其中的作用。方法 清洁级Wistar大鼠 80只 (雌雄各半 )给予不同浓度的酒精溶液(10 % ,30 % ,5 0 % )灌胃。于实验第 13周末 ,断头处死大鼠 ,分离血清和胰腺分别检测血清中血糖、胰岛素、IL - 1β、TNF -α和IL - 6以及胰腺组织中IL - 1β、TNF -α和IL - 6水平。 结果 5 0 %酒精雌雄大鼠组血清胰岛素水平降低 ,空腹血糖升高 ,血清IL - 1β、TNF -α、IL - 6水平升高 ,与正常对照组相比均有显著性差异 (P <0 0 5 ) ;胰腺组织中 ,5 0 %酒精雌性大鼠组IL - 1β、TNF -α、IL - 6和雄性大鼠IL - 1β、TNF -α水平升高。雄性大鼠IL - 6在各组间无显著性差异。结论 IL - 1β、TNF -α、IL - 6可能参与了长期过量酒精摄入而引起的胰岛 β细胞功能障碍。

Objective To investigate the effects of interleukin-1 bet a(IL-1β),tumor necrosis factor-alpha(TNF-α) and interleukin-6(IL-6) induced by chronic alcohol administration on pancreatic islets in rats.Methods Rats were given alcohol solution(6?mg/kg·d) in different concentrations(10%,30% and 50%) through intragastric-feeding for 13 we eks.The serum glucose,insulin and the level of IL-1β,TNF-α and IL-6 were deter mined at the end of the study.Results Compared with the control group,serum glucose in 50% alcohol feeding,was increased and insulin was decreased( P<0.05).The level of serum IL-1β,TNF-α and IL-6 were higher(P<0.05 ).Chronic alcohol consumption also elevated the level of IL-1β,TNF-α and IL-6 in pancreas of female rats and the level of IL-1β and TNF-α in pancreas of mal e rats(P<0.05).The content of IL-6 in pancreas had no differences in the f our groups of male rats(P>0.05).Conclusion Chronic alcohol intake can impair insulin secr etion function of beta cells.Moreover,chronic alcohol intake can up-regulate the production of cytokines(IL-1β,TNF-α and IL-6).In a word,chornic alcohol intox ication may have effect on beta cells through cytokines.