GA_3、2,4-D、B和蔗糖对梨花粉生活力及花粉生长速度的影响(英文)

Pollen Viability of Asian Pear and Effect of PGR, B and Sucrose on Germination and Pollen Tube Development

以5个亚洲梨品种为试材,通过离体培养研究了花穗中花的着生部位,不同浓度的GA3、2,4-D和蔗糖对花粉生活力、花粉管生长速度的影响。结果表明,不同品种的花粉生活力差异明显,黄花花粉萌发快;新世纪花粉生活力强,花粉管生长迅速。花的着生位置及花器官的发育程度对花粉生活力有明显影响,边花的生活力比中心花强。不同浓度的B、2,4-D、GA3对花粉萌发及花粉管生长均有不同程度的影响,高浓度的GA3、2,4-D抑制花粉管的生长,但浓度较高的蔗糖能促进花粉粒的萌发和生长,且两者呈正相关(R2=0.983)。

The pollen viability of 5 cultivars of Asian pear, the influence of flower position in a cluster and the effect of GA3, 2,4-D, B and sucrose on its germination and tube development were studied in vitro. The difference of viability among 5 cultivars was significant. The pollen of Huanghua germinated fast. Both germination rate and pollen tube length of Shinseiki were much higher and longer. Nijisseiki had the poorest performances on germination. Huanqing showed a low capability on pollen tube development; the length was the shortest within the 5 varieties. The flower position in a cluster and developmental stage influenced pollen viability; the viability of pollen grain from outer flowers of a cluster was greater than those from central flowers. Mediate concentration of B, GA3 and 2,4-D increased germination and pollen tube growth. The germination rate and pollen tube length on the medium that contained 15 mg/L of boron were greater than those of other treatments. There were negative relationship trend between pollen viability and the content of GA3 and 2,4-D. High content of sucrose on the medium benefited germination and pollen tube development. There was a linear regression relationship between germination rate and content of sucrose in the medium (R2=0.983).