摘要
用地高辛对胚胎发生晚期丰富蛋白LEA3基因片段(1.0 kh)进行了探针标记。斑点杂交表明,该探针的检测灵敏度为5 Pg。用地高辛标记和未标记的标准分子量DL 2000进行杂交时,只检测到2.00、1.00、0.75、0.50 kb4条杂交带。应用LEA3探针对转基因草莓(Fragaria ananassa Duch.)进行了Southern blot分析,检测到1.0 kh杂交带,表明外源LEA3基因整合到草莓染色体上。
Plasmid DNA probe of late embryogenesis abundant protein gene, LEA3 (1.0 kb), was labeled by digoxigenin. Dot blot showed that the probe was sensitive, able to detect 5 pg homologous DNA. Four hybridization bands (2.0 kb, 1.0 kb, 0.75 kb and 0.5 kb) were detected in Southern blot analysis of marker DL2000. The LEA3 probe labeled by digoxigenin was used in Southern blot analysis of transgenic strawberry (Fragaria ananassa Duch.) and hybridization bands were detected. The result indicated that LEA3 gene was integrated into the genome of strawberry.
出处
《果树学报》
CAS
CSCD
北大核心
2004年第4期331-333,共3页
Journal of Fruit Science
基金
河北省自然科学基金资助项目(302110)。
关键词
地高辛
LEA3基因
探针标记
转基因草莓
<Keyword>Digoxigenin
LEA3 gene
Probe label
Transgenic strawberry
