增生性瘢痕组织中TNF-α mRNA的动态表达及临床意义

The dynamic expression of TNF-α mRNA of hypertrophic scars and its roles

目的 从基因水平探讨肿瘤坏死因子 (TNF α)在增生性瘢痕组织发生、发展过程中的作用 ,为增生性瘢痕的基因治疗探索一条有效途径。方法 以正常瘢痕为对照 ,利用RT PCR技术分别检测 1～ 3个月、4～ 6个月、7～ 12个月、1年以上增生性瘢痕组织成纤维细胞中TNF αmRNA的表达情况。结果 随着增生性瘢痕组织成熟 ,TNF αmRNA表达的相对比值呈阶段性递增且差别明显 (P <0 0 1) ,与正常瘢痕比较含量仍明显为低 (P <0 0 5 )。结论 提示在正常伤口愈合过程中TNF α的作用甚为重要 ,增生性瘢痕的形成可能由于TNF α在组织中的含量降低有关。用基因治疗的方法提高早期增生性瘢痕中TNF α的含量可能为预防增生性瘢痕发生的一条有效途径。

Objective To investigate the role of tumor necrosis factor alpha:TNF-α in the hypertrophic scar and find a valid way to treat the hypertrophic scars with gene therapy. Methods TNF-α mRNA expression was tested with a semiquantitative reverse polymerase chain reation (RT-PCR) method in the different period of a hypertrophic scar. Results There was a significant increase of TNF-α mRNA as the hypertrophic scar was getting mature ( P <0.01) . However, the ratios of TNF-α mRNA was significantly in a lower level than the normal scar. Conclusions The results suggest that TNF-α may play an important role in normal wound healing, but the hypertrophic scar may result from the shortage of the TNF-α. This indicates that to increase the TNF-α with gene therapy may be a good way to treat the hyphertrophic scar .