葡多酚对胰腺细胞增殖活性的影响作用

Effect of grape procyanidin on proliferation of mice pancrease cell

目的 观察葡多酚 (GPC)对正常胰腺细胞和受损伤胰腺细胞增殖活性的影响。方法 ( 1)将经口灌胃不同剂量GPC的小鼠分作给予和不给予60 Co γ射线辐射 2种处理 ,取胰腺用MTT法检测细胞的增殖活性。 ( 2 )将加入和不加入GPC培养的胰腺细胞再分别加入双蒸水、葡萄糖和H2 O2 共同培养 2 4h ,测定细胞增殖活性。结果 ( 1)15 0mg/kgGPC组动物照射组和不照射组的细胞增殖率分别为 ( 3 16± 0 13) %和 ( 12 0 + 0 10 ) % ,均较不给GPC组显著性升高 (P <0 0 1)。 ( 2 )双蒸水、30mmol/L葡萄糖和 2 0mmol/LH2 O2 组细胞不加GPC的增殖活性分别为0 12 0± 0 0 0 8,0 0 70± 0 0 0 2和 0 0 6 3± 0 0 0 6 ,均较加入 10 0 μg/mlGPC显著降低 (P <0 0 5 )。结论 GPC可促进正常胰腺细胞的增殖活性 ,抑制辐射。

Objective To study the effect of grape procyanidin (GPC) on the proliferation of normal and injured pancrease cell.Methods (1) In vivo experiment,differet dose of GPC was given by mouth to the mice who radiated and not radiated by 60 Co -γ-ray,then proliferation of pancreas cells was measured by MTT assay.(2) In vitro experiment,The pancreas cells of mice were pretreated and not pretreated by GPC and then were cultrured with diferent concentration of glucose and H 2O 2 respectively for 24 hours.The proliferation of normal and injured pancreas cell was determined by the same assay.Results (1)Cell proliferation rate of radiation group and not radiation group given 150?mg/kg GPC were(3 16±0 13)% and (12 0±0 10)% respectively,higher than that of groups without GPC.(2)The cell proliferation of H 2O 2,30?mmol/L glucose and 2 0?mmol/L H 2O 2 groups not pretreated by GPC were 0 120±0 008、0 070±0 002 and 0 063±0 006 respectively,lower than that in groups pretreated by 100?μg/ml GPC.All the differences above were significant in statistics.Conclusion GPC can promote the proliferation of the normal pancreas cells and had protection on the proliferation of pancreas cells injured by radiation,glucose or H 2O 2