槲皮素在苯并芘致小鼠DNA损伤中的作用

Effect of quercetin on benzo[a]pyrene-induced DNA damage in mice

目的 研究槲皮素在苯并芘致小鼠DNA损伤中的作用。方法 小鼠一次性腹腔注射苯并芘 (B〔a〕P) 10 0mg/kg后 ,分别以 0 5 ,1 0 ,2 0g/ (kg·d)剂量的槲皮素连续灌胃 90d。取小鼠尾部静脉血进行单细胞凝胶电泳实验 ,通过荧光显像的方法观察并测量血细胞的拖尾长度。结果 阴性对照组小鼠的细胞无拖尾现象 ,高、中、低剂量槲皮素组和苯并芘阳性对照组的细胞均有拖尾现象。对彗尾进行统计学分析 ,与对照组相比 ,各剂量组均有显著性差异 (P <0 0 5 )。染毒组用不同剂量的槲皮素处理后 ,DNA的损伤程度显著减少。

Objective To study the effect of quercetin on DNA damage induced to benzo[a]pyrene in mice.Methods Male and female ICR mice received one ip injection of benzo[a]pyrene(100?mg/kg),then were fed with quercetin at the doses of 0.5,1.0,2.0?g/(kg·d) for three months.The single cell gel electrophoresis(SCGE) was used to study the DNA damage of mouse blood cell.Results Mice treated with benzo[a]pyrene had cometlength,whereas normal mice had no cometlength.After being treated with high,middle and low doses of quercetin for 3 months,the ratio of DNA damage and tail movement showed significant difference compared with the control( P <0 05),which showed significant dose-effect relationships.Conclusion Quercetin can prevent DNA damage from benzo[a]pyrene.