摘要
研究背景及目的 :组织低温保存后产生的效应包括细胞间和细胞质的改变 ,这些转变被细胞骨架系统所介导。本实验目的旨在研究皮肤低温储存后表皮层 β -肌动蛋白的变化 ,从而分析细胞骨架的低温生物学效应。材料和方法 :从同一个体取标本后用 5种不同的温度保存 ,分为新鲜组、4℃组、 - 2 0℃组、 - 80℃组和 - 196℃组 ,并在保存 7天、14天、2 1天后进行实验观察 ,实验采用两种不同的方法 :首先运用免疫组织化学染色和HE染色 ,从形态学上观察 β -actin的改变 ,并用图像分析仪测定表皮组织中的含量变化 ;继之 ,采用透射电镜观察微丝的变化并进行定量分析研究。研究结果 :两项实验得到相同的结果 ,即随着保存时间的延长 ,4℃组、 - 2 0℃组、 - 80℃组的 β -actin表达均较新鲜组 (P <0 . 0 5 )下降 ,而 - 196℃组与新鲜皮肤组表达相似。结论 :本实验表明皮肤组织的低温损伤与细胞骨架密切相关 ,β
The reaction of tissues to cryopreservation involves alterations in cell-cell and cell-matrix interactions mediated by cellular cytoskeleton. The aim of this study was to investigateβ-actin of epidermis for the analysis of hypothermal effects. MATERIAL AND METHODS: Two independent experiments were performed with samples from the same individual.These samples were preserved by 5 methods: fresh、4℃、-20℃、-80℃、-196℃. The exprements were carried out after 7 days, 14days and 21days. First of all, immunohistochemistry technology was adopted on the skin. Different changes have taken place on these groups. Furthermore, the influence of cryopreservation on β-actin was investigated in the skin tissue through electron microscope. RESULTS: We got the same result that alpha-tubulin was negatively regulated in both experiments. The group of 4℃、-20℃、-80℃ induced an downregulation of β-actin expression by the time go on. CONCLUSION: Consequently, these results demonstrate that the cryoinjury of skin is correlated with cytoskeleton, β-actin play important role in the mechanism of hypothermia preservation.
出处
《中国体视学与图像分析》
2004年第3期156-159,共4页
Chinese Journal of Stereology and Image Analysis
基金
国家自然科学基金资助 (No .30 0 70 2 98)
