RP－HPLC法同时测定血浆中阿普唑仑和多虑平

Simultaneous RP-HPLC Determination of Alprazolam and Doxepin in Plasma

本文首次采用反相ＨＰＬＣ法同时测定人血浆中阿普唑仑和多虑平。血浆样品在碱性条件下用乙酸乙酯提取，有机相在氮气下吹干，残渣用５０μｌ甲醇溶解，ＨＰＬＣ流动相为甲醇－０．０５ｍｏｌ／Ｌ醋酸铵缓冲液（内含１．０％三乙胺，ｐＨ５．０）（６０：４０），在２５４ｎｍ进行检测。阿普唑仑的线性范围为０～１００μｇ／Ｌ，ｒ＝０．９９８５，平均回收率为７７．３％，ＲＳＤ为３．２％～６．２％；多虑平的线性范围为０～５００μｇ／Ｌ，ｒ＝０．９９５７，平均回收率为９７．２％，ＲＳＤ为３．１％～６．２％。阿普唑仑和多虑平的最低检出限分别为１．２ｎｇ和４ｎｇ。本法简便、快速、灵敏、准确，适用于阿普唑仑和多虑平血药浓度的同时监测及临床药理学研究。

HPLC method for simultaneous determination of alprazolam (Alp) and doxepin (Dox) in plasma was described using a reversed phase column, packed with SPHERI-5 RP 18 (5μm) and UV Detection at 254nm. These drugs were extracted from 1ml of plasma at pH 13 into ethyl acetate, evaporated and reconsitituted in methanol. The latter was chromatographed using methanol- 0. 05mol/L ammonium acetate buffer containing 1. 0% triethylamine (pH 5. 0) (60 : 40) as mobile phase, at a flow rate of 1.0ml/min.The absolute recoveries and were 77. 3%, 3. 2￣6. 2% for Alp, and 97. 2% 3. 1 ￣6. 2% for Dox. The results were linear for Alp over the range 0￣ 100μg/L and for Dox over the range 0￣500μg/L. The detection limit was 1. 2ng for Alp and 4ng for Dox. This method is rapid and easy to handle with a conventional extraction procedure and satisfactory sensitivity and accuracy. This method is suitable in the clinical pharmacological research and TDM for Alp and Dox.