比色法测定维生素B_1片含量的研究

Study on Determimation of the Content of Vitamin B_1(Thiamine)by Colorimetric Method

本文实验比较了某些现有光度测定ＶＢ１方法，提出用盐酸苯肼－亚硝酸钠试剂与维生素Ｂ１反应生成橙红色溶液，进行光度测定，对比度△λ为９１ｎｍ，灵敏度，ε（４９７ｎｍ）＝１．１×１０４Ｌ·ｍｏｌ（－１）·ｃｍ（－１），在０～１０μｇ／ｍｌ范围内线性关系良好。用本法测定维生素Ｂ１片的含量，标准加入回收率范围为１００％～１０３％，片剂测定与中国药典１９９０年版紫外分光光度法测定结果一致。

After comparing experimentally some exiting spectrophotometric methods for the determination of vitamin B_1, a new, better method has been proposed, hased on the formation of an orange red solution by reaction between vitamin B_1 and pheny lhy drazine hydrochloride-sodium nitrite reagent solution. The contrast is 91nm, molar absorptivity 1. 1 × 10￣4L ·mol￣(-1) · cm￣(-1). Beer's law is obeyed for up to 10μg/ml of vitamin B_1. The method is simple, rapid, accurate and applicable to analysis of vitamin B_1 tablet with recoveries from 100% to 103%, the results of the aasays of tablets compare favourably.