反相高效液相色谱法测定血浆中左旋四氢巴马汀浓度

RP-HPLC Determination of l-Tetrahydropalmatine in Plasma

本文建立了测定血浆中左旋四氢巴马汀浓度的反相高效液相色谱法。采用国产ＹＷＧ－Ｃ（１８）填充柱，以甲醇－水（７０∶３０）为流动相，安定作内标，在紫外检测器２８１ｎｍ波长处进行监测，按内标法定量。血样用二氯甲烷提取。最低检测浓度为０．０６μｇ／ｍｌ，线性范围０．６２５～４０μｇ／ｍｌ，日内日间测定的ＲＳＤ小于６．９％。

A reversed-phase high performance liquid chromatographic method was developed for the determination of l-tetrahydropalmatine in plasma using a chemical bonded phase column (YwG-C_(18), 10μm) with diazepam as the internal standard. The mobile phase was a mixture of methanol-water solution (70∶30)with a flow rate of 0. 7ml/min. l-Tetrahydropalmatine was detected by UV absorption at 281 nm. To 200μl plasma sample, 100μl carbonate buffer (0. 2mol/L, pH10) and 20μl intemal standard solution were added, then extracted with 2ml dichloromethane. The organic layer was removed and evaporated to dryness under a nitrogen stream. The residue was dissolved in 50μl mobile phase and 10～25μl was injected into the chromatograph. Standard curve was linear in the range of 0. 625 to 40μg/ml. Tlie detection limit in plasma was 0. 06μg/ml. Analytical recoveries were 95～ 107%, the RSD of within-day and day-to-day were less than 6. 9% .