维生素B_6的流动注射分光光度法分析研究

Study on the Flow Injection Spectrophotometric Method for the Determination of Vitamin B_6

本文基于维生素Ｂ_６与醋酸钠和氯亚胺基－２，６－二氯醌的显色反应的原理，建立了维生素Ｂ_６的流动注射分析法，并对主要影响因素进行了探讨。当载流流速为１．５ｍｌ／ｍｉｎ，进样量为２００μｌ，反应管（ＩＤ０．８ｍｍ）长为４００ｃｍ，醋酸钠和氯亚胺基－２，６－二氯醌的浓度分别为２０％和０．５％时，其线性测量范围为３～６０μｇ／ｍｌ，回归方程为ｙ＝０．００９７＋０．０１８５ｃ，ｒ＝０．９９９７，用于复方制剂中维生素Ｂ_６的分析，平均回收率为９９．７％，ＲＳＤ为０．８％，样品分析结果同法定方法吻合。本文还采用停流反应分析技术对该显色反应的反应速度常数和反应产物的分解速度常数进行了测试。在测定条件下，反应速度常数为１．４７×１０^（－２）ｓ^（－１），分解速度常数为２．０３×１０^（－３）ｓ^（－１）。

A new method for the determination of vitamin B_6 by using flow injection spectrophotometry was studied. The main factors which affect the determination were investigated. Under optimum conditions, the calibration graph is linear in the range of 3￣60μg/ml, the recovery is 99.7% with a relative standard deviation of 0.8%. The method has been applied to the determination of Vitamin B_6, and their results are in good agreement with those obtained by classical method.