摘要
用荧光法对ＥＡＣ及ＥＡＣ／ＡＤＲ细胞内ＧＳＨ含量进行测定，发现ＥＡＣ／ＡＤＲ细胞内ＧＳＨ含量较ＥＡＣ细胞明显增高，分别为１．２５±０．３５及０．３１士０．１２μｇ／１０６细胞；但若对ＥＡＣ／ＡＤＲ细胞株停用ＡＤＲ治疗３６周后，则能使该细胞株部分恢复其敏感性，且ＧＳＨ水平也随之下降。另外，长春新碱、丝裂霉素Ｃ、放线菌素Ｄ及ＶＰ－１６对ＥＡＣ／ＡＤＲ细胞内ＧＳＨ水平均无明显影响。提示：ＧＳＨ水平增高，可能是多药抗药性产生的机理之一。
Determination of GSH was performed
by modified fluorometric method.Theexperiments showed that the GSH
content in resistant cell line(EAC/ADR)was 4 fold higher than thatin
sensitive cell line(EAC),being 1. 25士0.35μg/106 cells in EAC/ADR
and 0.31士0.12μg/106cells in EAC, When the drug treatment was
discontinued for 36 weeks,the sensitivity was partiallyrecovered and
the GSH level in EAC / ADR(R)cell parallelly decreased. In addition ,
vincristine,mitomycin C ,actinomycin D and VP-16 showed no effect on
the GSH content in the EAC/ADR cell.
出处
《药学学报》
CAS
CSCD
北大核心
1994年第1期20-23,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
关键词
阿霉素
抗药性
谷胱甘肽
抗癌药
Adriamycin
Cross
resistance
Multidrug resistance
Glutathione
