用（＋）－FLEC手性试剂自动柱前衍生高效液相色谱法拆分麻黄碱类药物对映体

RESOLUTION OF ENANTIOMERS OF EPHEDRINES USING (+)- FLEC CHIRAL DERIVATIZATION AND REVERSED-PHASE LIQUID CHROMATOGRAPHY

报道了以（＋）－ＦＬＥＣ作为手性衍生化试剂，用反相高效液相色谱技术分离麻黄碱类药物对映异构体的方法。ｄｌ－麻黄碱、ｄｌ－伪麻黄碱、ｄｌ－去甲麻黄碱、ｄｌ－去甲伪麻黄碱先与（＋）－ＦＬＥＣ形成衍生物后，经过一ＯＤＳＨｙｐｅｒｓｉｌ柱分离，以水和乙腈为流动相梯度洗脱．二极管阵列检测器在２６６ｎｍ检测。该法从样品衍生化到最终报告全部自动完成。上述各对对映体均达到基线分离，分离度Ｒ均大于１．５，方法灵敏度高，最低检出限达１０ｐｍｏ１，保留时间和峰面积的上现性较好，ＲＳＤ分别为１．１／和３．０％。

An automatic and rapid reversed phase liquid chromatographic method for the resolution of enantiomers of four ephedrines using (+)- FLEC as on-line pre-column chiral derivatizing reagent has been devcloped.After dl-ephedrine,dl-pseudoephedrine,dl-norephedrine and dl-norpseudoephedrine were derivatized with the chiral reagent(+)- FLEC [1-( fluorenyl ) ethylchloroformate] ,the resulting diastereomers were separated by using an acetontrile-water gradient elution on an ODS Hypersil column .This method was fully automated from sample derivatization to final report. Four enantiomersof ephedrines were well separated with resolution better that 1.5 . Thesensitivity was 1 0 pmol and reproducibility better than 1.0% RSD for retention time and 3 .0%fopeak area.