摘要
建立了反相高效液相色谱法测定人血浆中乙吗噻嗪浓度。色谱柱采用ＳｐｈｅｒｉｓｏｔｂＣ１８柱（２５ｃｍ×４．６ｍｍ，５μｍ），流动相为甲醇—水—三乙胺（７０：３０：０．４，ｐＨ６．５），检测波长２６８ｎｍ。用乙腈沉淀蛋白后，吹干浓缩进样。血药浓度在２０～４０００ｎｇ／ｍｌ范围内呈线性关系，相关系数０．９９９４，血浆最低检测浓度３ｎｇ／ｍｌ。方法回收率９０～１０３％，日内、日间ＲＳＤ２．４～１０．２％。应用该法研究了８名志愿者口服乙吗噻嗪片后的药代动力学，用一室模型拟合，消除相半衰期为１．７５±０．４５ｈ。本法简便、回收率和灵敏度高、重复性好，适于临床药代动力学和药效学的研究。
Following detailed study,a rapid and sensitive assay
for ethmozine in humanplasma has been developed using reversed phase
high performance liquid chromatography(HPLC).Plasma samples were
prepared for analysis by addition of internal
standard(5-chloro-2-amino-benzophenone )followed by protein preci
pitation using acetonitrile.A nalytical column was a C 18 spherisorb.
The mobile phase consisted of mathanol water triethylamine(70
:30:0.4,v/v/v,pH6.5).The column effluent was monitored at 268 nm. The
calibration curve was linear in the rangefrom 20 ng/ml to 4000 ng/ml
with r=0. 9994.The detection limit of this method was 3
ng/ml.Themethed showed good precision and the analytical recovery of
ethmozine from plasma was 90～105%;The relative standard deviations
for within- day and between- day were 2.4～6;3%and 4.5～
10.2%respectively.The plasma drug concentration- time course in man
after oral administration of 400 mgafter conformed to a 1-compartment
open model with a first order absorption phase.Mean T1/2valuewas
1.75+0.45
出处
《药学学报》
CAS
CSCD
北大核心
1994年第3期232-236,共5页
Acta Pharmaceutica Sinica
