HPLC柱切换法对炔诺酮α，β－聚（3－羟丙基）－DL－天冬酰胺在兔体内释放炔诺酮的测定

COLUMN SWITCHING HPLC METHOD FOR DETERMINATION OF IN VIVO RELEASE OF NORETHINDRONE-α,β-POLY(3-HYDROXYPROPYL)-DL-ASPAKAMIDE CONJUGATE

以柱切换ＨＰＬＣ（ＣＳＨＰＬＣ）技术，对炔诺酮α，β－聚（３－羟丙基）－ＤＬ天冬酰胺在家兔体内释放液中炔诺酮的血药浓度进行了测定，以自制ＯＤＳ预柱，水为流动相联机净化、富集样品；以Ｓｈｉｍ－ｐａｃｋＣＬＣ－ＯＤＳ为分析柱，甲醇－水（７：３）为流动相。结果表明ＣＳＨＰＬＣ对炔诺酮血药浓度有良好的选择性，回收率为８２．６％（ｎ＝５），日内和日间精密度（ＲＳＤ）分别小于４％和６％。在血清０．２２～１．１０μｇ／ｍ１范围内炔诺酮色谱峰面积与浓度有较好的线性关系（ｎ＝５，ｒ＝０．９９８）．

An HPLC method foY the determination of in vivo release of norethindrone-α,β-poly(3-hydroxypropyl)-DL -asparamide(NET- PHPA )conjugate was established using column switch-ing technique. The sample prepurification could be omitted. It was extracted by a precolumn(ODS,9～11μm)and the analytical column was packed with ODS(15 cm× 4 mm ID, Shimpac CLC).Themobile phases were water and methanol一water(7:3) respectively. The experiment showed that therecovery from serum was 82.6%,the RSD within day was l.3～1.8%and that in day一to一daywas 0.7～5.6%(n=4).