摘要
高效液相色谱法测定血浆中2-(对-二甲氨基苯乙烯)碘化甲基吡啶浓度李进文,曾平,张克锦(兰州军区乌鲁木齐总医院药理科830000)2-(对-二甲氨基苯乙烯)碘化甲基吡啶[2-(p-dimethylaminostyryl)pyridinemethiod...
A reversed phase high performance liquid
chromatography(RP-HPLC)methodfor determination of 2-(
p-dimethylaminostyryl) pyridine methiodide(DSPM)concentration in
rabbitplasma is reported.The stationary phase was YWG一C18 H37.The
mobile phase consisted of 80%CH3OH,20%CH3COOH一CH3COONa buffer(pH
4.0,0.20 mol/L)and(C2H5 )2NH 25mmol/L.Its flow rate was l.0 ml/min.
The column temperature and pressure was 28 ℃ and l
0.13MPa,respectively.The detect wavelength was 4 6 0 nm, The
detection limit of DSPM was 2 ng or0. 08umol/L in plasma, The
RSD(%)of within-day and between~days were less than 7%,A good1inear
relationship between peak area and DSPM concentration in plasma
(0.68~13;65u mol/L)wasfound(r=0. 9996)。
出处
《药学学报》
CAS
CSCD
北大核心
1994年第4期316-319,共4页
Acta Pharmaceutica Sinica
关键词
碘化甲基吡啶
高效液相色谱
血浆
HPLC
2-(
p-dimethylaminostyryl)pyridine methiodideDepartment of Pharmacology ,
XiNjiang Medical College ,Urumqi 830054