43株流感病毒型别鉴定及其特征分析

Type Identification and Characteristic Analysis on Influenza Virus for 43 Strains

[目的 ]了解 2 0 0 2年福州市区分离的 43株流感毒株型别特征。[方法 ]以MDCK和鸡胚法分离病毒 ,经红细胞凝集活性 (HA)和红细胞凝集抑制 (HI)试验鉴定分型。[结果 ]从 80 6份流感样病人的咽拭中分离出 43株流感病毒 ,其中 41株从MDCK法分离。在 2 2株A3型中 2 1株是“O”相毒株 ,其HI效价均在 1∶3 2 0以上 ,其中 0 2 411株效价 1∶64 0。B型 2 0株中类似B/沪防 / 2 0 / 2 0 0 1型 4株 ,类似B/浙江 / 2 / 2 0 0 1型 16株 ,其HI效价均≥ 1∶64 0。[结论 ] 2 0 0 2年福州市区流感病毒以A3型为主 ,B型次之 ,且A3型抗原性已发生较大变异 ;WHO于 2 0 0 3年 10月将以 0 2 411株为代表的A3型 (A3 /福建毒株 / 411/ 0 2 )作为 2 0 0 4年南半球流感疫苗组分推荐株。

Objective\] To investigate the type characteristics of influenza virus of 43 strains in Fuzhou in 2002 \ Influenza viruses were isolated by MDCK and egg inoculation method The types were identified by the test of hemagglutination activity (HA) and hemagglutination inhibition (HI) \ 43 strains of influenza virus were isolated from 806 pharynx swab in which 41 strains were separated by MDCK method 21 strains were ”O” phase virus in 22 strains of Type A3 and the titer of HI test was over 1∶320 in which the titer of “02411” strain was 1∶640 4 strains were similar to “B/shanghai/20/2001” and 16 strains similar to “B/zhejiang/2/2001” in 20 strains of Type B and the titer of HI were ≥1∶640 \[Conclusion\] The main influenza virus is Type A3, then is Type B in Fuzhou in 2002 and the antigenicity of Type A3 varied a lot In Oct 2003, ”02411” strain of Type A3 (”A3/Fujian viruses strains/411/02”) is identified as representative strains by WHO for the component of influenza vaccine in the southern hemisphere in 2004