摘要
用柱切换ＨＰＬＣ法建立了豚鼠血浆和皮下组织液中诺氟沙星银体内主要解离物诺氟沙星的测定方法。预处理柱为μ－ＢｏｎｄａｐａｋＣ１８，３７～５０μｍ，５０ｍｍ×５ｍｍＩＤ；分析柱为ＹＷＧ－Ｃｉ８，１０μｍ，１５０ｍｍ×５ｍｍＩＤ。预处理流动相为０．００８ｍｏ１／Ｌ磷酸缓冲液，流速为３ｍｌ／ｍｉｎ；分析流动相为甲醇—０．００８ｍｏｌ／Ｌ磷酸缓冲液—０．０５ｍｏｌ／Ｌ四丁基溴化铵（２５：７５：４）。紫外检测波长为２８０ｎｍ。皮下组织液及血浆中的线性范围分别为１００～３２００ｎｇ／ｍｌ（ｒ＝０．９９９９）和２～１２８μｇ／ｍｌ（ｒ＝０．９９９９）；最低检测浓度分别为１０ｎｇ／ｍｌ和０．２５μｇ／ｍｌ，方法的平均回收率为１０２％，日内及日间偏差均小于７％。
A column switching HPLC method
was established for the determination ofnorfloxacin, and active
dissociation product of silver norfloxacin ,in guinea pig plasma and
tissues.The pre-treating column(50 mm×5mm ID)and analytical column(
150 mm×5mm ID )werepacked with μ-Bondapak C18 ,37～50μm and YWG-
C18, 10μm,respectively. A mixture ofCH3OH 0. 008 mol/L phosphate
buffer .05 mol/L tetrabutylammoniun bromide(25 :75 :4)was used as
the analytical mobile phase with a flow rate of 1 ml/min. The
pre-treating mobile phasewas 0.008 mol/L phosphate buffer with a flow
rate of 3 ml/min. UV detection was made at 280 nm.The results were
linear within a range of 2～128μg/ml norfloxacin for plasma,The
lowest detectionlimit was 0.25μg/ml for plasma. The mean recovery of
the method was 102%with a relativestandard deviation of less than 7%.
出处
《药学学报》
CAS
CSCD
北大核心
1994年第7期539-543,共5页
Acta Pharmaceutica Sinica
