摘要
反相高效液相色谱法测定牛黄类中成药中胆汁酸的含量倪坤仪，王建，陈健，郁建，屠树滋（中国药科大学２１０００９）含牛黄的中成药种类很多，在医疗中具有广泛的用途。中药牛黄中主要成分为胆汁酸和胆红素。本文主要研究用ＨＰＬＣ法测定牛黄以及含牛黄中成药中胆汁酸的...
A
high performance liquid chromatograbphic method was established for
the deter-mination of cholic acid and hyodeoxycholic acid in bezoar,
Liushen pills ,Liuying pills and Niuhuang Xiaoyan pills. Bile acids
were labelled with 1-bromoacetylnitrobenzene in acetonitrile using
18-crown- 6 as catalyst. Derivatized bile acids were separated and
determined on uBondapak C18column (3.9mm×300mm,8￣10um),using
methanol-acetonitrile -water(24:35:41) as mobile phase and mandrolone
phenylpropionate as internal standard. Standard .Selection of
condition of derivatization reaction, mobile phase optimization,
determination of linearity range and check of intervenient factor
have been made.The accuracy of the method was checked by recoveries.
出处
《药学学报》
CAS
CSCD
北大核心
1994年第8期629-633,共5页
Acta Pharmaceutica Sinica
关键词
中成药
牛黄类
胆汁酸
药物分析
Reversed phase HPLC
Liushen pills:Liuying pills: Niuhuang
Xiaoyan pills: Bile acids
