苦地丁中异喹啉生物碱的反相离子对高效液相色谱分析法

DETERMINAT1ON OF ISOQUINOLINE ALKALOIDS IN CORYDALIS BUNGEANA TUREZ BY REVERSED PHASE ION-PAIR HIGH PERFORMANCE LIQUID CHROMATOGRAGHY XY

建立了苦地丁中４种主要生物碱（普洛托品、右旋紫堇灵、四氢黄连碱和乙酰紫堇灵）的反相离子对高效液相色谱分析法。用ＴＳＫ－１２０Ａ固定相，用含０．１％ＳＤＳ（ｐＨ８．０）的０．０２５ｍｏｌ·Ｌ－１磷酸二氢钠—甲醇（３５：６５）为流动相。方法简便、灵敏，并用此方法分析比较了４种不同产地和６种不同种类的苦地丁。

A simple and precise method using ion-pair high permance liquid chromatogra-phy was developed for the simultaneous determination of four isoquinoline alkaloids ,namelyprotopine,corynoline,acetylcorynoline and tetrahydrocoptisine in Corydalis bungeana Turez. A reversedphase system consisting of a chemically bonded ODS silica gel column and 0.025 mol·L-1 sodiumdihydrogen phosphate──methanol(35 :65)containing 0.1%sodium dodecyl sulphate(pH 8.0 as themobile phase was used.The four alkaloids were completely separated within 30 min. The analyticalresults for various samples were presented.