固相萃取结合高效液相色谱法测定人血浆中多索茶碱浓度

Determination of Doxofylline in Plasma by SPE-HPLC

目的:建立固相萃取结合高效液相色谱法测定人血浆中的多索茶碱浓度,为多索茶碱的体内血药浓度监测和人体药物动力学研究提供测定方法。方法:血样经固相萃取法处理,276nm紫外检测法测定,内标法定量。结果:平均回收率为82.5%,回归方程为Y=7.68×103X-2.00×103,r=0.9993。日内与日间RSD均低于10%。结论:该法重复性好、干扰小,是一种较为理想的测量方法。

Objective: To establish a method to determine doxofylline concentration in plasma by SEP-HPLC. Method: The doxofylline concentrations in plasma through solid-phase extraction were determined by HPLC method. Result: RSD of intra-day and inter-day were less than 10% the accuracy accorded with the plasma concentration monitoring and pharmacokinetic study. Conclusion: The SPE-HPLC method to determine doxofylline concentration is a ideal method.