对氨基联苯-H_2O_2-HRP分光光度法测定HRP的研究

Determination of horseradish peroxidase by spectrophotometry based on 4-aminobiphenyl-H_2O_2-HRP system

提出了对氨基联苯 -H2 O2 - 辣根过氧化物酶 (HRP)催化动力学光度法测定HRP的新方法。在辣根过氧化物酶的催化作用下 ,H2 O2 氧化对氨基联苯生成有色的偶氮联苯化合物 ,通过分光光度计在波长 4 5 5nm处测其吸光度值 ,可间接测定HRP的含量。在所选定的实验条件下 ,测定HRP的线性范围为 3. 0× 10 -10 ～ 8 .0× 10 -8g/mL ,检测限为 3. 0× 10 -10 g/mL。

A new method for the determination of horseradish per ox idase (HRP) was investigated by spectrophotometry based on 4-aminobiphenyl-H 2O 2-HRP system.It was based on H 2O 2 oxidation of 4-aminobiphenyl catalyz ed by HRP.When it was used in the determination of free HRP,the linear range was 3.0× 10 -10 ～8.0×10 -8g/mL,and the detection limit was 3.0× 10 -10g/mL.