恙虫病焦痂涂片检查方法的建立和应用

Establishment and application of eschar smear method for detection of ts utsugamushi disease

目的建立恙虫病焦痂涂片检查方法,并研究其在临床诊断中的应用. 方法使用Macchiavello染色法检测临床105例标本,同时作外斐氏试验OXk抗体检测.结果检查105例临床标本,结果有89例(84.76%)阳性.发热7d与14d以内焦痂涂片检查阳性结果差异无显著性(P＞0.05).发热7d内涂片检查阳性率明显高于OXk抗体滴度阳性率(P＜0.01);发热8～14d两法差异无显著性(P＞0.05).随发热天数的增加,阳性率开始呈下降趋势,同时OXk抗体滴度阳性率确有所增高.结论本法结果明确、迅速、方便.检出阳性率高.焦痂涂片与外斐氏试验对照检查可以提高诊断阳性率.可以作出恙虫病立克次氏体的初步(组别)报告.

Objective To establish eschar smear method for dete ct ion of tsutsugamushi disease and study its application in clinical diagnosis of the disease. Methods Macchiavello was performed on 105 clinical specimens and detected for Oxk antibody by Weil-Felix. Results Eighty-nine patients (84.76%) out o f the 105 donations was smear positive. No significant differences were observed in patients having a feverish duration of 7 and 17 days (P>0.05). The smear positive rate in patients having a feverish duration of within 7 days was highe r than that of Oxh antibody positive level. There were no significant difference s between the two methods (P>0.05). The positive rate was decreased along w ith the increase of feverish days ,while the positive rate of OXk antibody titer was increased. Conclusion This methods is rapid , accurate and simple and it is also as sensitive as Weil-Felix for diagnosis of the tsutwsugamushi disease.