磁性阿霉素隐形脂质体对裸鼠移植性人胃癌的靶向治疗

Targeting therapy of magnetic doxorubicin stealth liposome on human gastric cancer xenografts in nude mice

目的 探讨磁性阿霉素隐形脂质体靶向治疗裸鼠移植性人胃癌的疗效。方法 荷人胃癌的裸鼠为动物模型,尾静脉给药,肿瘤部位放置恒定磁场进行磁导向化疗。结果 磁性阿霉素隐形脂质体靶向MDL(+)组对人胃癌的移植瘤有显著抑制作用,体积和瘤重抑瘤率分别为71%和70 % ,显著高于磁性阿霉素隐形脂质体非靶向MDL(- )组(5 7%、5 0 % )和游离阿霉素DOX组(33%、31% )(P <0 .0 5 ) ,空白组细胞凋亡率均低于各实验组,MDL(+)组达4 2 % ,高于其他各组(P <0 . 0 5 )。实验期间DOX组体重一直未见增加,而MDL(+)组体重平稳增加,且饮食、活动未见明显变化。结论 磁性阿霉素隐形脂质体作为一种新型抗肿瘤药物显示了良好的应用前景。

Objective To study the effect of magnetic doxorubicin stealth liposome on human gastric cancer xenografts in nude mice.Methods Human gastric cancer cell line MKN-45 was implanted into 36 nude mice.Different kinds of drug were injected through the caudal vein of tumor bearing nude mice divided into 6 groups .Permanent magnet was put into tumor in targeting group.Results The growth speed of tumor in the group of MDL (+) significantaly slowed down than other groups.The rate of tumor restrain in tumor weight and tumor volume of MDL (+) group were 71% and 70%, which were remarkably higher than those of the DOX and MDL (-) group (all P <0.05).The body weight of nude mouse in DOX group failed to increase, while that in MDL (+) group increased steadly.Conclusion Magnetic doxorubicin stealth liposome as a new anti-tumor drug carrier may have a favorable clinical prospect.