肝细胞癌组织中DNA损伤修复基因APE1表达意义

Expression of apurinic/apyrimidinic endonuclease 1 in hepatocellular carcinoma

目的:探讨脱嘌呤/脱嘧啶核酸内切酶(apurinic/apyrimidinic endonuclease,Apel),又称氧化还原因子(redox fac—tor-1,Ref-1)在肝细胞性肝癌(HCC)的表达特点及其与临床病理因素的关系. 方法:应用免疫组化S-P法分别检测正常肝组织10例、结节性肝硬化组织40例和HCC103例组织中Ape1表达情况. 结果:在HCC组织中Ape1在细胞核、细胞质均可表达, 其中细胞核/细胞质联合表达(49.5%)显著高于结节性肝硬化组(20%)和正常对照组(0%)(P<0.01).Apel细胞核和细胞质阳性分度在正常组、肝硬化组、HCC组之间依次增高,并与HCC组织学分级有密切关系(P<0.01或0.05). 结论:癌细胞过度表达Ape1蛋白可作为判断肝癌组织学分级的有用指标之一.Ape1基因在HCC的发生、发展中可能具有重要作用,

AIM: To explore the expression and clinicopathological relevance of apurinic/apyrimidinic endonuclease (Ape1) in hepatocellular carcinoma (HCC). METHODS: Ape1 expression was detected by immu-nohistochemical S-P method in tissues of normal liver (n = 10), hepatocirrhosis (n = 40) and HCC (n = 103). RESULTS: Three types of Ape1 positive staining were noticed in HCC: nuclear, cytoplasmic and mixed. There were significant more mixed type of Ape1 expression in HCC than in hepatocirrhosis and normal liver (49.5% vs 20%, 0%, P<0.01). The positive degree of Ape1 expression in both nucleus and cytoplasm was significantly higher in HCC than that in hepatocirrhosis and normal liver, and higher in hepatocirrhosis than that in normal liver (P<0.01). The positive expression of Ape1 was correlated with the histological grade of HCC (P<0.05). CONCLUSION: Overexperssion of Ape 1 in neoplastic cells might be a useful marker in evaluating histoiogicai grade of HCC. Ape1 gene may play an important role in tumori-genesis and progression of HCC.