体液内毒素及深部真菌快速定量检测方法

Establishing a rapid method to quantitatively measurethe bacterial endotoxin and fungus content in plasma of infectious patients

目的:应用MB-80微生物快速动态检测系统,建立快速体液内毒素及深部真菌定量检测方法. 方法:在无菌条件下,留取20例正常人、10例严重腹腔革兰阴性菌感染及5例深部真菌感染病人的血浆,经前处理后,用MB-80系统动态浊度定量法,1 h完成检测.内毒素检测采用鲎试验动态浊度法,通过检测真菌细胞壁上1-3-β-D-葡聚糖的含量来反映深部真菌的水平.经计算机光电检测动态浊度变化,计算出内毒素及真菌含量. 结果:本法操作快速、简便,前处理过程标准化,结果重复性好,两种物质的检测可以同时在一台仪器上进行.正常人血浆内毒素和真菌水平在(2.03±2.32)ng/L和(2.46±2.15)ng/L,符合试剂盒提供的正常值范围(均<10 ng/L).严重感染病人不同时相点血浆内毒素含量范围在50～3 958 ng/L.深部真菌感染病人血浆真菌含量范围在20～ 2 381 ng/L. 结论:应用MB-80微生物快速动态检测系统,可以定量检测体液内毒素及真菌实际含量,作为临床上早期难以确诊的内毒素血症和深部真菌感染的辅助性诊断手段.

Objective: Using MB-80 Microbiology Kinetic Rapid Reader, the methods were established to quantitatively measure the body fluid content of bacterial endotoxin and fungus in infectious patients. Methods: The blood samples were collected from Gram negative bacterial infection and deep mycosis patients and healthy volunteers. After pre-treatment, the plasma samples were measured using MB-80 system through kinetic turbidimetric limulus test method. The system computer could calculate the content of endotoxin and fungus during 1 hour detection. Results: The detection methods of endotoxin and fungus using MB-80 were simple and rapid. The process of pre-treatment was standardized and the results had good repeatability. The normal ranges of plasma endotoxin and fungus of human healthy volunteers were (2.03±2.32)ng/L and (2.46±2.15)ng/L respectively. The plasma endotoxin level of abdominal infection patients was increased significantly to 50-3 958 ng/L. The fungus level of deep mycosis patients was enhanced to 20-2 381 ng/L. Conclusion: The methods of endotoxin and fungus quantitatively measurement by MB-80 are simple and convenient and have results with good repeatability. The new detection methods of endotoxin and fungus can be used for diagnosis of endotoxemia and deep mycosis in early stage of infection.