拉米夫定联合胸腺肽α1治疗鸭乙型肝炎的效果观察

Combination of lamivudine with thymosin α1 in treatment of duck hepatitis B

目的探讨联合应用胸腺肽α1(Tα1)和拉米夫定对樱桃谷鸭乙型肝炎病毒(DHBV)复制的抑制作用。方法以DHBV阳性血清感染1日龄樱桃谷鸭,制备鸭乙型肝炎模型。用拉米夫定治疗12w后,联用Tα1治疗8d,以拉米夫定治疗为对照组,半定量PCR法检测鸭血清中DHBV,常规病理学方法观察鸭肝组织病理变化情况。结果和生理盐水对照组比较,拉米夫定治疗后鸭血清中DHBV水平显著降低(4483.2±5193.4vs9351.8±5059.6),联合Tα1治疗后抗DHBV效果更加明显(1692.2±589.2)。拉米夫定治疗可减轻肝细胞变性(3.2±0.8vs4.6±0.5)和炎症反应程度(6.2±3.3vs8.6±2.8),联合Tα1治疗后肝脏炎症反应有所加强(9.0±5.2)。结论拉米夫定可抑制DHBV复制,联用Tα1抗病毒效果更好,而且有加强肝脏内免疫功能的作用。

Objective: To explore the inhibitory effect of combination of lamivudine with thymosin α1 (Tα1) on the replication of duck hepatitis B virus (DHBV). Methods: Peking ducks of 1 d old were challenged with DHBV-positive serum and used as a duck hepatitis B model.After treated with lamivudine for three months,the ducks were randomly grouped and treated with or without Tα1 for 8 d.Serum DHBV titrate was observed by semi-quantitative PCR,and inflammation and degeneration of hepatocytes were observed by pathology examination. Results: The serum DHBV titrate was significantly reduced (4483.2±5193.4 vs 9351.8±5059.6) after lamivudine treatment,and it was reduced more significantly(1692.2±589.2) after combination treatment with Tα1.Lamivudine reduced the degeneration degree of hepatocytes (3.2±0.8 vs 4.6±0.5) and the inflammation degree of liver (6.2±3.3 vs 8.6±2.8). The combination treatment with Tα1 increased liver inflammation degree (9.0±5.2). Conclusions: Both Tα1 and lamivudine may reduce the replication of DHBV in Peking ducks and combination treatment may have the better anti-virus effect and enhance immune response in liver. [