GSTM1基因多态与HBV相关性肝细胞癌易感

Association of genetic polymorphisms in glutathione S-transferases M1 with hepatitis B-related hepatocellular carcinoma

目的探讨谷胱甘肽转硫酶(GSTs)M1基因多态性与乙型肝炎相关性肝细胞癌及其与HBV感染引起的慢性乙型肝炎和肝硬化的相关性。方法采用病例-对照流行病学研究方法,选取了91例原发性肝癌(HCC)患者、58例肝硬化(LC)患者、63例慢性乙型肝炎(CHB)患者和134例正常对照者。以多重PCR技术,检测GSTM1缺失情况。结果正常组GSTM1基因缺失率(46.92%)与HCC组(61.53%)差异有显著性(P<0.05),与LC组(45.61%)、CHB组(49.18%)差异无显著性。携带GSTM1空白基因型者比非空白基因型者患原发性肝癌的风险高1.81倍(OR=1.81,95%CI=1.05～3.12)。携带的55岁前发病的HCC病人GSTM1空白基因型分布明显较55岁后发病者高(P<0.05)。结论GSTM1空白基因型与肝癌易感性有关,且与其发病年龄有关,与肝硬化和慢乙肝无明显相关。

Objective: To investigate the association of genetic polymorphisms in glutathione S-transferases(GST) M1 with hepatitis B-related hepatocellular carcinoma (HCC). Methods: Genomic DNA was isolated from peripheral blood of HBsAg carriers,including 91 cases of HCC,58 liver cirrhosis(LC),63 chronic hepatitis B(CHB),and 134 normal controls.GSTM1 genotypes were detected by multiplex PCR. Results: The null genotype of GSTM1 was significantly frequent in patients with HCC compared with controls (P<0.05),but there were no significant differences in frequency of GSTM1 null genotype among patients with liver cirrhosis,chronic hepatitis B and normal controls.Subjects carrying null genotypes of GSTM1 had higher risk of developing HCC compared with those carrying positive genotype (OR=1.81,95% CI=1.05~3.12). Conclusion: The GSTM1-null genotype may be associated with an increased risk of HCC,but not of CHB and LC. [