大肠杆菌T蛋白的调节结构域的定位分析

Mapping of regulatory domain of T-protein from Escherichia coli

目的分析大肠杆菌T蛋白是否拥有独立的调节结构域,并确定其在氨基酸序列上的定位。方法利用分段克隆法克隆了11个大肠杆菌T蛋白的片段,检测各片段的调节活性。结果发现大肠杆菌T蛋白的调节结构域定位于C末端约270个氨基酸,并与其预苯酸脱氢酶结构域密不可分。结论大肠杆菌T蛋白没有独立的调节结构域。

Objective: To map the regulatory domain of Escherichia coli T-protein. Methods: Fragmentation cloning was employed in cloning of 11 fragments from T-protein.The regulatory activity of each fragment was determined respectively. Results: The regulatory domain of T-protein was located in the C-terminal 270 amino acids,which was the same location as PDH domain. Conclusion: T-protein has no independent regulatory domain. [