磁性阿克拉霉素纳米粒的研制和理化性质

Preparation and Characterization of Magnetic Aclacinomycin-A Nanoparticles

目的 制备纳米级磁性阿克拉霉素微粒 ,考察其理化性质和磁场响应性。方法 运用化学沉淀法制备Fe3 O4纳米颗粒 ,并与阿克拉霉素水溶液分散在正己烷油相体系。采用反相微乳液界面聚合的方法生成聚合物将水液滴包埋 ,由此制备内含磁性颗粒和阿克拉霉素的磁性纳米载药系统 ,并通过扫描电镜、透射电镜、动态光散射磁强计等考察磁性阿克拉霉素纳米粒的理化性质及体内外磁场响应性。结果 磁性阿克拉霉素纳米粒平均粒径2 1 0nm ,阿克拉霉素载药率 1 0 .0 %,包封率 36 .2 %。制备过程中的各参数包括单体用量、体系油水相体积比例、有机溶剂的种类和乳化剂的用量等都会影响磁性阿克拉霉素纳米粒粒径和阿克拉霉素包封率。制备后 4℃保存 ,3个月稳定性好。结论 磁性阿克拉霉素纳米粒粒径小 ,载药率和包封率高 ,体外具有良好的磁场响应性。

Purpose The objective of this study is to prepare magnetic nanoparicles for drug delivery system with polybutylcyanoacrylate(PBCA)and aclacinomycin A (ACM-A). Methods Magnetic PBCA nanospheres loaded with ACM and Fe 3O 4 nanoparticle were prepared by interfacial polymerization and reverse-phase microemulsion.The structure of this polymer,loading rate and embedding ratio were confirmed by SEM,TEM,DLS and vibrating-sample magnetometer(VSM). Results The drug's loading rate was 10.0%,embedding ratio was 36.2% and the mean particle size was 210 nm.Various parameters including amount of monomer,organic solvent,the kind of surfactants、ratio of oil/water phase and reaction temperature,could affect the particle size and the entrapment of the M-ACM-PBCA-NP. Conclusions M-ACM-PBCA-NP responded well to the applied magnetic field and M-ACM-PBCA-NP was an efficient new carrier of ACM by magnetic mechanisms.