血管内皮生长因子在大鼠心脏移植急性排斥期的表达

Expression of vascular endothelial growth factor during acute rejection in rats' cardiac transplantation

目的 观察血管内皮生长因子(VEGF)在大鼠心脏移植急性排斥期中的表达及其和排斥的关系。方法 大鼠分对照组,CSA组,每组12只。分别静脉给予生理盐水及CSA干预,采用颈部心脏异位移植术式建立移植模型。常规监测排斥反应发生情况。每组5只用于观察移植物存活时间,7只用于动态切取标本。应用逆转录 聚合酶链反应(RT PCR)检测移植物局部VEGF的表达水平。结果 CSA组移植心存活时间(2 0 .4±5 .1)d显著长于对照组(8.6±1.5 )d ,CSA组VEGF的表达强度弱于对照组,其时间变化趋势和高峰时间较对照组推迟并和移植心存活延长时间有相关性。病理观察显示移植心局部的炎性细胞和淋巴细胞浸润和VEGF表达有关。结论 VEGF高表达促进排斥反应的发生缩短移植心存活时间。

Objective To study the expression of vascular endothelial growth factor (VEGF) during acute rejection in rats' cardiac transplantation.Methods Allografts were treated by CSA (CSA group, n = 12) or normal saline (control group, n = 12) post operation immediately.Allograft samples were obtained separately on the day 1,3,5,7,9,11,14 post operation.The local expression of VEGF was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).Results The survival time of cardiac allografts was significantly prolonged in CSA group (20.4± 5.1) days as compared with that in control group (8.6± 1.5) days.The expression level of VEGF was decreased in CSA group as compared with control group.Conclusion The high-level expression of VEGF has proinflammatory functions in accelerating the rejection of allograft.