壳聚糖及衍生物与大鼠脑的生物相容性研究

Biocompatibility of chitosan and its derivative in rat brains

目的 探讨壳聚糖及衍生物在大鼠脑内的生物相容性,筛选出适宜的药物载体。方法 将壳聚糖及衍生物混悬液注射入脑内,分别在3、7、14、3 0d检测血清神经元特异性烯醇化酶(NSE)和S 10 0蛋白水平,同时作病理学检查,并用生理盐水作对照。结果 第3、7、14、3 0天壳聚糖组NSE和S 10 0蛋白水平与生理盐水组比较差异均无统计学意义(P >0 .0 5 ) ,高黏度壳聚糖组几乎均高于生理盐水组(P <0 .0 5 ) ,且在7、14d高于壳聚糖组(P <0 .0 5 )。羧甲基壳聚糖组NSE水平7d高于壳聚糖组(P <0 .0 5 ) ,14d高于生理盐水组(P <0 .0 5 )。光镜下壳聚糖组反应与生理盐水组类似,高黏度壳聚糖组与羧甲基壳聚糖组胶质细胞增生明显,早中期有脑水肿,且降解迟缓。结论 壳聚糖对神经系统损害轻微,具有良好的生物相容性。

Objective To evaluate biocompatibility of chitosan and its derivative in rat brains,so as to screen adequate drug vehicle.Methods The suspension of chitosan and its derivative was injected into rat brains,then the levels of serum NSE and S-100 protein were measured at 3,7,14,30 days.Pathological examination was performed simultaneously,using saline as control.Results There was no significant difference in the levels of NSE and S-100 protein between chitosan group and saline group at 3,7,14,30 days ( P > 0.05). The levels of NSE and S-100 protein in hyperviscosity chitosan group were almost all higher than those of saline group ( P < 0.05), especially higher than that of chitosan group at 7,14 days ( P < 0.05). The levels of NSE in carboxymethly chitosan group were higher than those of chitosan group at 7 days ( P < 0.05), and higher than those of saline group at 14 days ( P < 0.05). The changes in chitosan group was similar to that of saline group under the light microscopy.However,the glial cells in hyperviscosity chitosan group and carboxymethly chitosan group proliferated obviously and had brain edema at early and middle period with slow degradation.Conclusion Chitosan has little damage to nervous system,and possesses fine biocompatibility.Carboxymethly chitosan has less efficiency.