河北省莱姆病媒介蜱的初步调查

Survey on Tick Vectors of Lyme Disease Spirochetein Hebei Province

我们于1992年5～9月份,在承德、保定、邯郸及石家庄地区采用布旗法及牲畜身上捕捉法共收集蜱208只,其中全沟硬蜱最多147只占71.01％,森林革蜱35只,长角血蜱25只,日本血蜱1只。从全沟硬蜱分离出3株莱姆病螺旋体:HIP_6HIP_8HIP_(11)。另外两种蜱未分离出莱姆病螺旋体。在暗视显微镜下,菌体呈细长疏螺旋状,以旋转、移动及侧曲方式运动,运动活泼。在直接免疫荧光试验中与免疫抗B_(31)免疫血清呈特异性免疫,在间接免疫荧光试验中与单克隆抗体H_(6724)及H_(5332)呈阳性反应,与H_(6831)呈阴性反反。

In 1992, we collected 208 ticks by flagging vegetation and from domestic animals in Chengde, Baoding, ShiJiazhuang and Handan. These ticks were 147 Ixodes persulcatus,35 Dermaconior psilvarvm,25 Haemaphysalis longic-ornis and 1 Haemaphy sails jaonica. Three strains of Borrelia burgdorferi were isolated from Ixodes persulcatus and no strain was isolated form other two species of ticks, the isolates were strongly reactive to the immunofluo-rescent antibody against B31 and to the monoclonal antibodies H5332, H6724 but not reactive to the H6831.