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家蚕磷酸甘油醛异构酶Tpi基因的克隆 被引量：5

Cloning of Triosephoshpate Isomerase Gene of the Silkworm,Bombyx mori
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摘要 利用生物信息学方法成功地克隆了家蚕磷酸甘油醛异构酶 (triosephosphate isomerase Tpi) 基因 (GenBank登记号为AY734490).该基因全长为2 875 bp;含有5 个外显子、4个内含子,所有内含子/外显子边界都符合典型的GT/AG剪切模式;cDNA 序列全长1 051 bp,并经RT-PCR克隆和序列分析进行了验证;具有完整的开放阅读框架(ORF,95～839 bp),可编码248个氨基酸的蛋白.通过与NCBI蛋白数据库的比对表明,该基因编码的蛋白为磷酸甘油醛异构酶(EC 5.3.1.1),参与葡萄糖分解代谢,即将磷酸二羟丙酮转化为3-磷酸甘油醛,在果蝇、蚊子、黄粉虫、美洲淤夜蛾、棉铃虫等昆虫中具有高度的保守性. A novel silkworm,Bombyx mori,triosephosphate isomerase(Tpi) gene is cloned by blasting its EST database and PCR test,and its sequence has been deposited in the GenBank (Accession No: AY734490).It has five extrons and four introns spanning 2 875 bp in full length and all the boundary of extrons/introns has GT/AG conserved sequences.Its 1 051bp mRNA has a complete open reading fragment (ORF: 95-834) and encodes 248 Aa protein,which is triosephosphate isomerase(EC 5.3.1.1)for catalysis of conversion dihydroxyacetone phosphate into glyceraldehydes 3-phosphate in glucose glycolysis and very conserved in many insects such as Heliothis virescens,Helicoverpa armigera,Tenebrio molito,Anopheles gambiae and Drsophila melanogaster.
作者 牛宝龙 孟智启 翁宏飙 沈卫锋 吕顺霖
机构地区 浙江大学动物科学学院 浙江省农业科学院蚕桑研究所
出处 《蚕业科学》 CAS CSCD 北大核心 2005年第1期47-52,共6页 ACTA SERICOLOGICA SINICA
基金 国家自然科学基金项目(编号30371087)。
关键词 EST数据库 PCR 磷酸甘油醛异构酶 家蚕 EST database PCR Triosephosphate isomerase Bombyx mori
分类号 S881.2 [农业科学—特种经济动物饲养] Q78 [生物学—分子生物学]
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参考文献17

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共引文献54

同被引文献59

  • 1沈卫锋,牛宝龙,翁宏飚,孟智启.利用EST数据库资源克隆家蚕Bombyx mori6-磷酸葡萄糖酸脱氢酶基因[J].蚕桑通报,2005,36(1):14-19. 被引量：4
  • 2金红建,邵健忠,项黎新.表达序列标签数据库搜索克隆斑马鱼QM基因及其数字化差异显示分析[J].水产学报,2005,29(5):599-605. 被引量：3
  • 3许鸿川,王经源,陈斌,王发明,林文雄,张健.常绿阔叶林优势种福建青冈RAPD反应体系的建立[J].福建农林大学学报（自然科学版）,2006,35(6):623-627. 被引量：5
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引证文献5

二级引证文献6

蚕业科学
2005年 第1期

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