抗菌多肽葛佬素的原核表达纯化及活性鉴定

Prokaryotic expression, purification and identification of anti-bacterial peptide gloverin

目的 原核表达抗菌肽葛佬素,并对其生物学活性进行初步鉴定。方法 应用pET原核表达系统进行目的 蛋白的表达,应用蛋白免疫印迹对蛋白进行鉴定,并采用鲎试剂法,对表达产物的生物活性进行初步鉴定。结果 体外表 达产物经蛋白免疫印迹鉴定,为融合有聚组氨酸标签的葛佬素抗菌肽,鲎试剂检测结果表明,该表达产物对LPS有明显的 中和活性。结论 在原核表达系统中成功表达了具有生物活性的抗菌肽葛佬素。

Objective To express anti-bacterial peptide gloverin in prokaryotic system and investigate its bioactivity. Methods E.coli BL21 was used to express gloverin. Western blot was used to identify the production and Tachypleus Amebocyte Lysate (TAL) was used to detect the bioactivity of the production. Results The production was identified as gloverin peptide by Western blotting, and the TAL data indicated that the production could neutralize LPS. Conclusion Anti-bacterial peptide gloverin is successfully expressed in E.coli BL21.