接种Aβ_(42)全肽疫苗恒河猴的特异性体液免疫应答

Specific humoral immuneresponse in Rhesus monkeys vaccinated immune response with Aβ_(42) peptide vaccine

目的: 观察恒河猴接种Aβ42肽疫苗后的特异性抗体的产生。方法: 将 5只雄性恒河猴分别在 0、2、6、10、14、18、22wk肌内注射Aβ42肽疫苗; 用ELISA法检测恒河猴血清抗Aβ42抗体水平及IgG亚类; 用Westernblot检测血清抗Aβ42抗体的特异性; 免疫组化染色法观察抗血清对Tg2576转基因小鼠脑组织中Aβ斑的识别。结果: 疫苗接种后第 8周, 恒河猴血清中出现明显的抗Aβ42抗体, 抗体水平随着接种次数的增加而升高, 第 24周达 1∶4 320, 以后抗体水平开始下降。产生的抗Aβ42抗体以IgG1和IgG2为主 (IgG2 /IgG1 >1)。血清抗Aβ42抗体具有高度特异性, 可识别Tg2576转基因小鼠脑组织中的Aβ斑。结论: Aβ42肽疫苗可有效地诱导恒河猴产生特异性体液免疫应答。

AIM: To observe the humoral immuneresponse in Rhesus monkey induced by Aβ 42 peptide vaccination. METHODS: Five male Rhesus monkeys received intramuscular injection of Aβ 42 peptide vaccine at 0, 2nd, 6th, 10th, 14th, 18th, 22th week. The titers and Ig subclasses of the serum anti-Aβ 42 antibody were measured by ELISA. The specificity of the anti-Aβ 42 antibody was determined by Western blot. The recognition of Aβ plaques in Tg2576-transgenic mouse brain tissue by anti-Aβ 42 serum were detected by immunohistochemical staining. RESULTS: At the 8th week after the vaccination, the serum anti-Aβ 42 antibody was detected. The titers of the antibody increased with times of booster inoculation and reached 1∶4 320 at the 24th week, then decreased. The produced antibodies were mainly IgG1 and IgG2(IgG2/IgG1>1). The anti-Aβ 42 antibody had high specificity. The Aβ plaques in Tg2576-transgenic mouse brain tissue were recognized by the antisera. CONCLUSION: Aβ 42 peptide vaccine can induce effectively specific humoral immuneresponse in Rhesus monkey.