摘要
本文报告咐醇酯(TPA)对25例不同型别原代白血病细胞的诱导分化作用。结果表明,TPA可诱导髓系白血病细胞向单核巨噬细胞方向分化,表现为细胞由悬浮转为贴壁,胞浆突起伸出丝状伪足,溶菌酶分泌增多,形态与细胞化学反应具有单核巨噬细胞特征。而 TPA对林巴白血病(急淋ALL和 B慢淋B—CLL)细胞的影响较少,无上述一系列变化出现。毛细胞白血病(HCL)在TPA作用下反应独特,发生比急性非淋巴细胞白血病(ANLL)更为迅速和显著的贴壁以及胞浆长枝状突起,细胞内抗酒石酸钾酸磷酶(TRAP)呈强阳性。TPA体外诱导分化实验,有助于了解白血病细胞的分化潜能,对于鉴别诊断形态学分型困难的ANLL与ALL,B—CLL与HCL具有十分重要的实用价值。
This paper reports the effect of 12 - 0 - tetradecanoylphorbol - 13 - acetate(TPA) on primary culture of leukemic cells from 25 cases of myeloid and lymphoidleukemias. It showes that TPA could induce differentiation of human myeloidleukemia cells into monocytie macrophagic pathway. The changes include adherenceof the cells to the surface of the culture dish, long filamentous Pseudopodia of thecytoplasm and increase of lysozyme activity in culture supernatants. Morphology andcytochemica stain of the cells are also with characteristics of macrophages. Butlymphoid leukemia cells are little affected by treatment with TPA and don't show theabovementioned series changes. Hairy cell leukemia showed special response to theTPA induction: The adherence and long filamentous pseudopodia of the cells areinduced more rapidly and remarkbly than ANLL and the activity of tartrate resistantacid phosphatase enhanced graduslly. These results suggest that TPA - induced differentiation test may provide amodel to study the differentiation potential of leukemic cells and it may be ofimportant value for distinguishing ANLL and ALL, B-CLL and HCl, especially inleukemias which are difficult to classify by morphologic method.
